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[1]赵彤,蒋跃利,闫浩,等.土壤氨化过程中微生物作用研究进展[J].应用与环境生物学报,2014,20(02):315-322.[doi:10.3724/SP.J.1145.2014.00315]
 ZHAO Tong,JIANG Yueli,YAN Hao,et al.Research advances on microbial function in soil ammonifying process[J].Chinese Journal of Applied & Environmental Biology,2014,20(02):315-322.[doi:10.3724/SP.J.1145.2014.00315]
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土壤氨化过程中微生物作用研究进展()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年02期
页码:
315-322
栏目:
综述
出版日期:
2014-04-25

文章信息/Info

Title:
Research advances on microbial function in soil ammonifying process
作者:
赵彤蒋跃利闫浩黄懿梅
1西北农林科技大学资源环境学院,农业部西北植物营养与农业环境重点实验室 杨凌 712100 2西北农林科技大学黄土高原土壤侵蚀与旱地农业国家重点实验室 杨凌 712100
Author(s):
ZHAO Tong JIANG Yueli YAN Hao HUANG Yimei
1 College of Natural Resource and Environment, Key Laboratory of Plant Nutrition and Agri-environment of Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling 712100, China 2 State Key Laboratory of Soil Erosion and Dry Land Farming on Loess Plateau, Northwest A&F University, Yangling 712100, China
关键词:
氨化过程氨化微生物碳氮比限速步骤高通量测序
Keywords:
ammonifying process ammonifier carbon to nitrogen ratio rate-limiting step high-throughput sequencing
分类号:
S154.34
DOI:
10.3724/SP.J.1145.2014.00315
文献标志码:
A
摘要:
土壤氮素矿化产生的无机氮是植物的主要氮素来源,土壤氨化过程是氮素矿化的第一步,微生物在其中发挥着巨大作用. 本文从氨化过程的微生物作用机理,可利用碳氮比、蛋白酶和微生物群落结构等影响因素以及微生物研究方法3个方面来讨论微生物对氨化过程的重要贡献. 研究发现高分子可溶性有机氮的解聚作用很可能是氨化过程的限速步骤,土壤微生物生物量氮有可能是微生物易利用氮的直接且主要来源,同时土壤可利用碳氮比对氨态氮的产生具有重要影响. 最后介绍了分子生物学新方法尤其是高通量测序技术在土壤微生物作用研究中的应用,并就目前未解决问题和今后研究方向提出展望.
Abstract:
Inorganic nitrogen which comes from soil nitrogen mineralization is the main nitrogen source for plant. Ammonifying process is the first step of nitrogen mineralization, in which microbes play an important role. This paper discussed the effects of microbes in ammonifying process from three aspects: the microbial action mechanism of degrading soil organic nitrogen into ammonia, the influencing factors including available carbon to nitrogen ratio, protease and microbial community structure, and some latest techniques for microbial research. Some researches found that the depolymerization of high molecular weight soluble organic nitrogen is likely the rate-limiting step in ammonifying process; soil microbial biomass nitrogen may be the direct and main source of microbial available organic nitrogen; at the same time, soil available carbon to nitrogen ratio has a significant effect on the production of ammonium. In the end, we introduced some new molecular biology techniques, especially high-throughput sequencing for research of soil microbes, and suggested the unsolved problems and possible future research direction.

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备注/Memo

备注/Memo:
国家自然科学基金重点项目(41101254)、西北农林科技大学基本科研费项目(QN2011020)和黄土高原土壤侵蚀与旱地农业国家重点实验室开放基金(K318009902-1321)资助
更新日期/Last Update: 2014-05-04