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[1]蒲祥,李光州,肖青,等.产喜树碱的喜树内生真菌Aspergillus sp. LY013的分离、退化及退化后的次级代谢产物[J].应用与环境生物学报,2013,19(05):787-793.[doi:10.3724/SP.J.1145.2013.00787]
 PU Xiang,LI Guangzhou,XIAO Qing,et al.Isolation, Attenuation, and Secondary Metabolites of Attenuated Camptothecin-Producing Endophytic Fungus Aspergillus sp. LY013 from Camptotheca acuminata[J].Chinese Journal of Applied & Environmental Biology,2013,19(05):787-793.[doi:10.3724/SP.J.1145.2013.00787]
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产喜树碱的喜树内生真菌Aspergillus sp. LY013的分离、退化及退化后的次级代谢产物()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年05期
页码:
787-793
栏目:
研究论文
出版日期:
2013-10-25

文章信息/Info

Title:
Isolation, Attenuation, and Secondary Metabolites of Attenuated Camptothecin-Producing Endophytic Fungus Aspergillus sp. LY013 from Camptotheca acuminata
作者:
蒲祥 李光州 肖青 易进海 田永强 张国林 赵立兴 罗应刚
(1中国科学院成都生物研究所 成都 610041) (2福建省高校森林生态系统过程与经营重点实验室 福州 350002) (3武夷学院生态与资源工程学院 南平 354300)
Author(s):
PU Xiang LI Guangzhou XIAO Qing YI Jinhai TIAN Yongqiang ZHANG Guolin ZHAO Lixing LUO Yinggang
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China) (2State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China) (3University of Chinese Academy of Sciences, Beijing 100049, China) (4Institute of Chinese Materia Medica, Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China) (5College of Chemical Engineering, Sichuan University, Chengdu 610065, China) (6Key Laboratory of Microbial Diversity in Southwestern China of Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China)
关键词:
喜树内生真菌喜树碱曲霉LY013
Keywords:
Camptotheca acuminata endophytic fungus camptothecin Aspergillus sp. LY013
分类号:
Q936 : Q948.12+2.3
DOI:
10.3724/SP.J.1145.2013.00787
摘要:
为寻找喜树碱产生菌,从喜树内皮中分离得到50株内生真菌。采用HPLC-DAD分析、筛选这些内生真菌的发酵产物,并进一步采用HPLC-DAD-HRMS确证喜树碱的产生。结果表明,其中一株编号为LY013的内生真菌具有产喜树碱的能力。根据LY013菌株的生长形态、菌丝体和孢子分析,将其鉴定为Aspergillus sp. LY013。在后续进行的传代培养研究中,发现LY013生产喜树碱的能力在传代过程中迅速退化。为了进一步了解LY013的化学成分背景,对该菌株的次级代谢产物进行了深入研究。从LY013的发酵产物中,共分离获得9个复杂生物碱。综合运用波谱方法和相关化学技术,将这9个复杂生物碱鉴定为pseurotin A(1)、FD-838(2)、fumitremorgin C(3)、tryprostatin B (4)、cyclotryprostatin B(5)、12,13-dihydroxyfumitremorgin C(6)、spirotryprostatin A(7)、fumiquinazoline J(8)和fumiquinazoline C(9)。上述结果为进一步研究Aspergillus sp. LY013奠定了基础。图2 参25
Abstract:
In search of camptothecin (CPT)-producing microorganisms, 50 endophytic fungi were isolated from the inner bark of a fully mature Camptotheca acuminata. These endophytes were fermented and the fermentation broth and mycelia were analyzed by HPLC-DAD for CPT-producing capability that was confirmed by HPLC-DAD-HRMS analysis. The isolate designated LY013 was found to produce CPT in the culture broth. The endophyte LY013 was determined to be Aspergillus sp. on the basis of the morphological analysis of mycelia and spores. Subsequent subculturing of LY013 led to a substantial decrease of the production of CPT. To understand the chemical background of the attenuated CPT-producing endophytic fungus Aspergillus sp. LY013, chemical investigations of the secondary metabolites of LY013 fermentation broth were performed. Nine complex alkaloids were isolated and identified as pseurotin A (1), FD-838 (2), fumitremorgin C (3), tryprostatin B (4), cyclotryprostatin B (5), 12,13-dihydroxyfumitremorgin C (6), spirotryprostatin A (7), fumiquinazoline J (8), and fumiquinazoline C (9) on the basis of the interpretation of spectral data and by comparison with the spectral data reported previously. Fig 2, Ref 25

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备注/Memo

备注/Memo:
中国科学院知识创新工程领域前沿项目、“西部之光”联合学者项目、生命科学领域优秀青年科技专项(KSCX2-EW-Q-6)和国家自然科学基金面上项目(21172216)联合资助 Supported by the Forefront Program, the “Western Light” Joint Project, the Science and Technology Project for Outstanding Youths in Life Science (No. KSCX2-EW-Q-6) from the Chinese Academy of Sciences, and the National Natural Science Foundation of China (No. 21172216)
更新日期/Last Update: 2013-10-28