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[1]李明,王树香,高宝嘉.不同群落类型油松居群的遗传多样性[J].应用与环境生物学报,2013,19(03):421-425.[doi:10.3724/SP.J.1145.2013.00421]
 LI Ming,WANG Shuxiang,GAO Baojia.Genetic Diversity of Pinus tabulaeformis Carr. Populations in Different Forest Types[J].Chinese Journal of Applied & Environmental Biology,2013,19(03):421-425.[doi:10.3724/SP.J.1145.2013.00421]
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不同群落类型油松居群的遗传多样性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年03期
页码:
421-425
栏目:
研究论文
出版日期:
2013-06-25

文章信息/Info

Title:
Genetic Diversity of Pinus tabulaeformis Carr. Populations in Different Forest Types
作者:
李明王树香高宝嘉
(1河北农业大学生命科学学院 保定 071000)
(2河北农业大学林学院 保定 071000)
Author(s):
LI MingWANG ShuxiangGAO Baojia
(1College of Life Sciences, Agricultural University of Hebei, Baoding 071000, China)
(2College of Forestry, Agricultural University of Hebei, Baoding 071000, China)
关键词:
油松ISSR遗传分化群落类型DNA水平多态性
Keywords:
Pinus tabulaeformis ISSR genetic diversity forest community types genetic polymorphism
分类号:
Q949.665.03 : S791.254.02
DOI:
10.3724/SP.J.1145.2013.00421
摘要:
探讨群落差异下油松居群适应性和变异特征对油松资源管理和利用具有重要意义. 采用ISSR分子标记方法检测我国特有树种油松居群的遗传变异,分析3个居群间的遗传分化水平,探讨影响群体遗传分化的主要因素和机制. 结果表明,13个ISSR引物共检测到185个可重复的位点,其中多态位点168个,总多态位点百分率(P)为90.81%,平均多态位点百分率为66.38%;3个油松居群间的遗传分化系数Gst平均为0.178 1,表明群体间的遗传分化水平较高,遗传变异82.19%存在于群体内,居群间的基因流(Nm = 2.3081)相对异交风媒植物(Nm = 5.380)较小;3个植物群落油松居群的Nei’s遗传多样性指数大小顺序为:油松+核桃楸+白桦混交林(YHB)>油松纯林(YY)>油松+华北落叶松+白桦混交林(YLB);居群间的遗传相似度平均为0.902 7;油松居群的遗传多样性与枝下高和辛普森指数呈显著正相关性;油松居群遗传变异与居群的群落特征关系密切. 不同群落类型生境影响油松遗传多样性水平.
Abstract:
It is important to make clear the adaptability and variation characteristics of the pine of different forest community types for pine resource management and utilization. In this paper genetic diversity and population genetic structure of 3 natural P. tabulaeformis Carr. populations in different forest types were studied by means of the newly developed inter simple sequence repeats (ISSR) marker. Based on the study of population genetic structure, the genetic differentiation and its impact factors were analyzed among the P. tabulaeformis populations. The results from 13 ISSR primers showed that 185 repetitive loci were produced in 83 individuals of P. tabulaeformis. Among the 185 loci, 168 (90.81%) were polymorphic. The average percentage of polymorphic loci of the three populations was 66.38%. The coefficient of genetic differentiation of the three P. tabulaeformis populations was 0.178 1. The mean of gene flow (Nm) was 2.308 1, relatively steady among populations, which meant reduced partial genetic variance and genetic differentiation. Furthermore, the gene flow was lower than that of outcrossing and anemophilous plant population. The order of Nei’s genetic diversity of the 3 populations was: mixed forest of P. tabuliformis + Juglans mandshurica + Betula platyphylla (YHB) > pure forest of P. tabuliformis (YY) > mixed forest of P. tabuliformis + Larix gmelinii + B. platyphylla (YLB). The mean of genetic identity among the 3 populations of P. tabulaeformis was 0.902 7. It suggested a significant positive correlation between the genetic diversity of Chinese pine populations and both under crown height and Simpson’s index, as well as the close relation between the population genetic variation of P. tabulaeformis and its community characteristics. The genetic polymorphism level of natural Chinese pine populations were influenced by different population habitat types.

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备注/Memo

备注/Memo:
河北省自然科学基金项目(C2008000231)资助
更新日期/Last Update: 2013-06-20