|本期目录/Table of Contents|

[1]李云,黄耀佳,王晓蓉.UV-B辐射下悬沙对小球藻生长和DNA损伤的影响[J].应用与环境生物学报,2012,18(04):605-609.[doi:10.3724/SP.J.1145.2012.00605]
 LI Yun,HUANG Yaojia,WANG Xiaorong.Effects of Suspended Solids on Growth and DNA Damage of Chlorella sp. Exposed to UV-B Radiation[J].Chinese Journal of Applied & Environmental Biology,2012,18(04):605-609.[doi:10.3724/SP.J.1145.2012.00605]
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UV-B辐射下悬沙对小球藻生长和DNA损伤的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
18卷
期数:
2012年04期
页码:
605-609
栏目:
研究论文
出版日期:
2012-08-25

文章信息/Info

Title:
Effects of Suspended Solids on Growth and DNA Damage of Chlorella sp. Exposed to UV-B Radiation
作者:
李云黄耀佳 王晓蓉
(1中国水产科学研究院东海水产研究所 上海 200090)
(2华东师范大学生命科学学院 上海 200062)
Author(s):
LI YunHUANG YaojiaWANG Xiaorong
(1East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai 200090, China)
(2School of Life Sciences, East China Normal University, Shanghai 200062, China)
关键词:
悬沙UV-B辐射小球藻细胞密度DNA损伤
Keywords:
suspended solids UV-B radiation Chlorella sp. cell density DNA damage
分类号:
Q949.2 : Q947.8
DOI:
10.3724/SP.J.1145.2012.00605
文献标志码:
A
摘要:
研究了UV-B辐射(17 ?W cm-2,5 min/d)下不同浓度(0、100和1 000 mg L-1)和粒径组成(全粒径组和粒径< 38 ?m组)的悬沙对小球藻(Chlorella sp.)生长和DNA损伤的影响. 结果显示,小球藻的生长可用Logistic增长模型拟合,拟合后的生长参数表明悬沙的“遮荫效应”减轻了UV-B辐射对小球藻细胞的DNA损伤,从而对小球藻生长产生正影响. 悬沙浓度越高,UV-B辐射对小球藻细胞DNA损伤程度越低,小球藻的环境负载能力a和瞬时增长率K越大. 当悬沙浓度为100 mg L-1时,全粒径组a值和K值小于粒径< 38 ?m组,且两组间小球藻细胞DNA损伤差异显著(P < 0.05),但当悬沙浓度达1 000 mg L-1时,全粒径组a值和K值大于粒径< 38 ?m组,两组间细胞的DNA损伤无显著差异(P > 0.05). 图3 表2 参24
Abstract:
Effects of suspended solids (SS) with different concentrations (0, 100 and 1 000 mg L-1) and different particle sizes (all size range and size < 38 ?m) on the growth and DNA damage of Chlorella sp. exposed to UV-B radiation (17 ?W cm-2, 5 min/d) were studied. The growth of Chlorella sp. was well predicted by a logistic model, and the growth parameters showed that, SS attenuated DNA damage induced by UV-B and promoted the cell growth. With the increase of SS concentration, the cellular DNA damage induced by UV-B decreased, and the environmental carry capacity (a) and instantaneous rate of increase (K) increased. When the cells were exposed to SS of 100 mg L-1, the values of a and K were lower in all-size-range group than in the group of size < 38 ?m, and a significant difference in the cellular DNA damage (P < 0.05) appeared between them. When exposed to SS of 1 000 mg L-1, a and K were higher in all-size-range group than in the group of size < 38 ?m, but no significant difference in the cellular DNA damage (P > 0.05) was found. Fig 3, Tab 2, Ref 24

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备注/Memo

备注/Memo:
国家重点基础研究发展规划项目(No. 2010CB429005)和中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院东海水产研究所)项目(No.2008M13)资助
更新日期/Last Update: 2012-08-21