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[1]叶冰莹,邱思,周平,等.甘蔗蔗糖磷酸合成酶SPSⅡ cDNA片段克隆与表达分析[J].应用与环境生物学报,2011,17(05):673-677.[doi:10.3724/SP.J.1145.2011.00673]
 YE Bingying,QIU Si,ZHOU Ping,et al.Cloning and Expression Analysis of Sucrose-phosphate Synthase II Gene from Sugarcane[J].Chinese Journal of Applied & Environmental Biology,2011,17(05):673-677.[doi:10.3724/SP.J.1145.2011.00673]
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甘蔗蔗糖磷酸合成酶SPSⅡ cDNA片段克隆与表达分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年05期
页码:
673-677
栏目:
研究论文
出版日期:
2011-10-24

文章信息/Info

Title:
Cloning and Expression Analysis of Sucrose-phosphate Synthase II Gene from Sugarcane
作者:
叶冰莹邱思周平宋喜梅陈由强陈如凯
(1福建师范大学生命科学学院,2农业部甘蔗生理生态与遗传改良重点开放实验室,3福建省农业科学院果树研究所 福州 350108)
(4中国热带农业科学院南亚热带作物研究所 湛江 524091)
Author(s):
YE BingyingQIU SiZHOU PingSONG XimeiCHEN YouqiangCHEN Rukai
(1College of Life Sciences, Fujian Normal University, 2Key Laboratory of Sugarcane Genetics and Breeding, Ministry of Agriculture, 3Fruit Research Institute, Fujian Academy of Agriculture Sciences, Fuzhou 350108, China)
(4South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, Guangdong, China)
关键词:
蔗糖磷酸合成酶基因克隆表达分析实时荧光定量PCR甘蔗
Keywords:
sucrose phosphate synthase gene cloning expression analysis real-time PCR sugarcane
分类号:
Q78 : S566.101
DOI:
10.3724/SP.J.1145.2011.00673
文献标志码:
A
摘要:
蔗糖磷酸合成酶(Sucrose phosphate synthase,SPS)是蔗糖合成途径的关键限速酶,在蔗糖积累和碳分配中具有重要作用. 在茎组织中SPSⅡ表达量占SPS转录总量的40%,表明SPSⅡcDNA的克隆与表达对蔗茎中蔗糖的积累有着重要的影响. 通过RT-PCR技术克隆分离SPSⅡ基因cDNA片段. 序列分析表明该cDNA片段包含1个3 183 bp的开放读码框,可编码1 060个氨基酸. GenBank登录号为EU269038. 通过实时荧光定量PCR检测SPSⅡ基因在甘蔗糖分积累的不同时期、不同组织部位的相对表达量. 结果表明,在糖分积累初期蔗茎中的SPSⅡ相对表达量最大,在糖分积累中期蔗叶中的SPSⅡ相对表达量达到最高峰;同组织部位中,糖分积累初期SPSⅡ相对表达量高于糖分积累中期、后期. 图6 参11
Abstract:
To achieve the ultimate aim of increasing sucrose content in the sugarcane stem, it is necessary to investigate the principal rate-limiting steps in the pathway of sucrose synthesis. It has been suggested that sucrose phosphate synthase (SPS) is a key rate-limiting enzyme in sucrose formation and it also influences sucrose accumulation and carbon partitioning processes. Relative expression of SPSⅡ gene occupies 40% of the total transcription of SPS gene, which shows that the cloning and expression of SPSⅡ gene influences sucrose accumulation process. This research focused on molecular cloning and expression analysis of SPSⅡ gene in sugarcane. The SPSⅡ cDNA fragment was isolated from sugarcane, which contained a 3 183 bp open reading frame (ORF), encoding 1 060 amino acids. Its Genbank accession no. EU 269038. Relative expression of SPSⅡ gene was determined in the different tissues of sugarcane at different time of sugar accumulation by real-time PCR. The results showed highest relative expression of SPSⅡ gene in the canes at early stage during accumulation process of sucrose, but highest in the leaves at middle stage. In the same photosynthetic tissue, relative expression of SPSⅡ was higher at early stage during accumulation process of sucrose than those at middle stage and later stage. Fig 6, Ref 11

参考文献/References:

1 Zhang MF (张明方), Li ZL (李志凌). Sucrose-metabolizing enzymes in higher plants. Plant Physiol Commun, 2002, 38 (3): 289~295
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3 Zhou P (周平), Ye BY (叶冰莹), Chen YQ (陈由强), Chen RK (陈如凯). The recent advances on sucrose phosphate synthase. Lett Biotechnol (生物技术通讯), 2006, 17 (6): 1001~1003
4 Liu NX (刘凌霄), Shen FF (沈法富), Lu HQ (卢合全), Han QD (韩庆点), Liu YG (刘云国). Research advance on sucrose phosphate synthase in sucrose metabolism . Mol Plant Breeding (分子植物育种), 2005, 3 (2): 275~281
5 Li W (李雯), Shao YZ (邵远志), Chen WX (陈维信). Research advances in the relationships of sucrose phosphate synthase (SPS) with fruit quality and maturity and senescence. Acta Bot Bor-occid Sin (西北植物学报), 2005, 11: 2340~2344
6 Yang GZ (杨国志), Zhang MF (张明方). 植物蔗糖代谢参与酶的表达及调控. 北方园艺, 2006, 5: 45~47
7 Castleden K, Naohiro A , Gillespie VJ, MacRae EA, Quick WP, Buchner P, Foyer CH, Furbank RT, Lunn JE. Evolution and function of the sucrose-phosphate synthase gene families in wheat and other grasses. Plant Physiol, 2004, 135 (3): 1753~1764
8 He WJ (何文锦), Huang JC (黄金存), Zhou P (周平), Ye BY (叶冰莹), Chen YQ (陈由强), Chen RK (陈如凯). Cloning and sequence analysis of sucrose phosphate synthase cDNA from sugarcane leaf . Subtrop Agric Res (亚热带农业科学), 2007, 3 (4): 294~296
9 Lutfiyya LL, Xu NF, Ordine RL, Morrell JA, Miller PW, Duff SMG. Phylogenetic and expression analysis of sucrose phosphate synthase isozymes in plants. Plant Physiol, 2007, 164 (7): 923~933
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11 Grof CPL, So CTE, Perroux JM, Bonnett GD, Forrester RI. The five families of sucrose-phosphate synthase genes in Saccharum spp. are differentially expressed in leaves and stem. Funct Plant Biol, 2006, 33: 605~610

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备注/Memo

备注/Memo:
现代农业产业技术体系建设专项资金和国家自然科学基金项目(No. 30871575)资助
更新日期/Last Update: 2011-10-25