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[1]曹哲明,杨健.不同浓度Cd2+对鲤鱼基因组DNA的影响[J].应用与环境生物学报,2010,16(04):457-461.[doi:10.3724/SP.J.1145.2010.00457]
 CAO Zheming,YANG Jian.Effect of Cadmium at Different Concentrations on Genomic DNA of Common Carps[J].Chinese Journal of Applied & Environmental Biology,2010,16(04):457-461.[doi:10.3724/SP.J.1145.2010.00457]
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不同浓度Cd2+对鲤鱼基因组DNA的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年04期
页码:
457-461
栏目:
胁迫抗性生理及分子机制专栏
出版日期:
2010-08-25

文章信息/Info

Title:
Effect of Cadmium at Different Concentrations on Genomic DNA of Common Carps
作者:
曹哲明 杨健
1南京农业大学无锡渔业学院 无锡 214081
2中国水产科学研究院淡水渔业研究中心内陆渔业环境与资源重点开放实验室 无锡 214081
Author(s):
CAO Zheming YANG Jian
(1Wuxi Fisheries College, Nanjing Agriculture University, Wuxi 214081, Jiangsu, China)
(2Key Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Centre, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China)
关键词:
鲤鱼DNA甲基化重金属污染
Keywords:
cad mium Jian common carp (Cyprinus carpio var. Jian) DNA methylation heavy metal pollution
分类号:
X174 : S917.4
DOI:
10.3724/SP.J.1145.2010.00457
文献标志码:
A
摘要:
鲤鱼(建鲤品种)幼鱼暴露于4个浓度组(0.005、0.05、0.5、5 mg/L)的镉离子(Cd??2+)2 d??,期间各组的个体均无死亡. 用10个RAPD引物对处理后鲤鱼基因组进行扩增,亦未发现有明显的差异扩增条带. 但在用甲基化敏感扩增多态性方法研究暴露后鲤鱼肌肉组织的基因组DNA甲基化的变化时发现,5 mg/L的Cd??2+在?? 2会使鲤鱼基因组DNA中CCGG区域甲基化发生明显的变化. 该组个体1 mo后出现大量死亡. 另外,0.05 mg/L和0.5 mg/L Cd??2+组个体基因组DNA中CCGG区域甲基化比例2 ??内略有下降,甲基化位点没有太大改变. 说明鲤鱼幼鱼基因组CCGG区域的胞嘧啶甲基化比例在不同浓度Cd??2+处理中都比较稳定. 低浓度Cd??2+条件下鲤鱼主要通过甲基化区域的微调减少Cd??2+的生理毒性. 高浓度Cd??2+处理下,利于基因组DNA甲基化区域发生相当的变化,可能导致一些沉默基因的异常表达. 图2 表2 参31
Abstract:
T he3-month-ol Jiancommoncarps(TThe 3-oldJian common carps (Cyprinus carpio var. Jian) were exposed to various concentrations of Cd 2+ (0.005, 0.05, 0.5, 5 mg/L) for two days, and no individuals died within the two-day exposure. Also, no significantly amplified loci were found in both treatment and control fishes when ten RAPD primers were detected for the difference in genome DNA. However, when methylation sensitive amplified polymorphism (MSAP) was applied to analyze methylation changes in genome DNA of muscle, significant changes of methylation in CCGG sites occurred in the fish after 2 days of 5 mg/L Cd2+ exposure. Furthermore, most fish in this group died within one month. In the groups exposed to 0.05 mg/L and 0.5 mg/L for two days, a slight decline in the proportion of methylation loci were observed, but no obvious change in methylation pattern in comparison with the controls. This study shows that the methyltion of Jian carp in CCGG sites of genome DNA was relatively stable during exposure to various concentrations of Cd 2+. At low Cd 2+ concentration the Cd 2+ physiological toxicity might be reduced by Jian common carp with fine-tuning in methylated sites, while at high Cd2+concentration, significant changes would occur in methylation sites of genome DNA, possibly leading to an abnormal expression of some silent genes. Fig 2, Tab 2, Ref 31

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备注/Memo

备注/Memo:
中央级公益性科研院所基本科研业务费专项资金(No. 2009JBFB022009JBFBBFB02)和人事部高层次留学人才回国工作项目(No. 2-115084)资助 Supporte Supportedby the Central Public-interest Scientific Institution Basal Research Fund ofChinaf China (No. 200 JBFB02)andtheMinistryofPersonnelofChina(No.2-1150 )
更新日期/Last Update: 2010-08-25