|本期目录/Table of Contents|

[1]刘慧芬,王卫卫,曹桂林,等.氢气对刺槐根际土壤微生物种群和土壤酶活性的影响[J].应用与环境生物学报,2010,16(04):515-518.[doi:10.3724/SP.J.1145.2010.00515]
 LIU Huifen,WANG Weiwei,CAO Guilin,et al.Effect of Hydrogen on Microbial Population and Enzyme Activity in Robinia pseudoacacia Rhizosphere Soil[J].Chinese Journal of Applied & Environmental Biology,2010,16(04):515-518.[doi:10.3724/SP.J.1145.2010.00515]
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氢气对刺槐根际土壤微生物种群和土壤酶活性的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年04期
页码:
515-518
栏目:
研究论文
出版日期:
2010-08-25

文章信息/Info

Title:
Effect of Hydrogen on Microbial Population and Enzyme Activity in Robinia pseudoacacia Rhizosphere Soil
作者:
刘慧芬王卫卫曹桂林唐明
(1西北大学西部资源生物与现代生物技术教育部重点实验室 西安 710069)
(2西北农林科技大学林学部 杨凌 712100)
Author(s):
LIU Huifen WANG Weiwei CAO Guilin TANG Ming
(1Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’ an 710069, China)
(2College of Forest, Northwest A & F University, Yangling 712100, Shaanxi, China)
关键词:
氢气根际土壤氢氧化细菌微生物代谢强度土壤酶根瘤固氮刺槐
Keywords:
hydrogen rhizosphere soil hydrogen-oxidizing bacteria microbial metabolism intensity soil enzyme nitrogen-fixation Robinia pseudoacacia
分类号:
S144 : S154
DOI:
10.3724/SP.J.1145.2010.00515
文献标志码:
A
摘要:
模拟豆科植物根瘤在固氮过程中释放氢气,分析其对根际土壤产生的影响. 从黄土高原人工刺槐林采集有根瘤的刺槐根际土壤样品,采用电解水产生氢气分次处理,对处理后与未处理的土壤样品内微生物种群数量、微生物代谢强度及土壤脱氢酶、过氧化氢酶、脲酶、转化酶等指标进行比较分析. 氢气处理后的两批土样中,氢氧化细菌数量均显著增多,分别占9月、5月土壤微生物总量的34.9%和32.4%;微生物在土壤中代谢强度分别增加了140.2%和57.6%;土壤脱氢酶、过氧化氢酶、脲酶和转化酶含量均表现增加,其中9月处理土壤分别增加36.6%、57.1%、26.6%和147.0%,5月处理土壤分别增加37.2%、6.6%、108.8%和147.0%. 研究结果为根瘤固氮过程中产生氢气对根际土壤微环境的影响及促生机制提供了实验资料. 图2 表3 参14
Abstract:
In the nitrogen-fixation process, hydrogen is released and affects plant rhizosphere soil. Soil samples containing locust nodules were collected from artificial Robinia pseudoacacia forest of the Loess Plateau in China in September 2008 and May 2009, and treated by hydrogen produced with electrolysis of water 30 days later. The number of soil microbial populations, microbial metabolisability, and soil dehydrogenase, catalase, urease and invertase contents were determined and analyzed. The results showed that the number of hydrogen-oxidizing bacteria increased distinctly in the treated soil, accounting for 34.9% in September and 32.4% in May of the total soil microorganisms. Microbial metabolism intensity in the treated soil was increased by 140.2% and 57.6% than that in the untreated soil. Dehydrogenase, catalase, urease and invertase contents in the treated soil were also increased, by 36.6%, 57.1%, 26.6% and 147.0% than those in the untreated soil in September 2008 and by 37.2%, 6.6%, 108.8%, 147.0% in May 2009. The results indicated the hydrogen produced during nitrogen-fixation would affect the rhizosphere soil micro-environment and the growth-promoting mechanism. Fig 2, Tab 3, Ref 14

参考文献/References:

1 Hesterman OB, Sheaffer EC, Beurns DK. Alfalfa dry matter and nitrogen production and fertilizer nitrogen response in leguminous-corn rotation. Agronomy, 1986, 78: 19~23
2 Irvine P, Smith M, Dong ZM. Hydrogen Fertilizer: Bacteria or Fungi? Acta Hort, 2004, 631: 239~242
3 Dong ZM, Wu L, Kettlewell B. Hydrogen fertilization of soils-is this a benefit of legumes in rotation. Plant Cell & Environ, 2003, 26: 1875~1879
4 Chen XD (陈兴都), Wang WW (王卫卫), Fu B (付博), Xiao LM (肖黎明), Guo Y (郭燕). Growth promoting effect of hydrogen-oxidizing bacteria in soybean rhizosphere. Acta Bot Bor-Occid Sin (西北植物学报), 2008, 28 (1): 136~140
5 Fu B (付博), Wang WW (王卫卫), Tang M (唐明), Chen XD (陈兴都). Isolation and identificantion of hydrogen-oxidizing bacteria producing 1-aminocyclopropane-1-carboxylate deaminase and the determination of enzymatic activity. Acta Microbiol Sin (微生物学报), 2009, 49 (3): 395~399
6 Meng QM (孟庆枚), Hua SZ (华绍祖), Yan WZ (阎文哲). Loess Highlands Conservation of Water and Soil. Zhengzhou, China: Yellow River Hydraulic Press (郑州: 黄河水利出版社), 1999. 96~132
7 Jiamila M, Zhang Y, Yang J. Isolation and characterization of hydrogen-oxidizing bacteria induced following exposure of soil to hydrogen gas and their impact on plant growth. Environ Microbiol, 2007, 9 (2): 435~444
8 Lu YH (陆雅海), Zhang FS (张福锁). The advances in rhizosphere microbiology. Soils (土壤), 2006, 38 (2): 113~121
9 Li ZG (李振高), Luo YM (骆永明), Teng Y (滕应). 土壤与环境微生物研究法. Beijing: Science Press (北京: 科学出版社), 2008. 102~128
10 Fu B (付博), Wang WW (王卫卫), Hao Y (郝莹), Wang LJ (王莉娟), Tang M (唐明), Chen XD (陈兴都). Isolation and identificantion of hydrogen-oxidizing bacteria in Medicago sativa rhizophere. Chin J Appl Environ Biol (应用与环境生物学报), 2009, 15 (5): 650~654
11 Li L, Li SM, Sun JH. Diversity enhances agricultural productivity via rhizosphere phosphorus facilitation on phosphorus-deficient soils. PNAS, 2007, 3 (104): 11192~11196
12 Liang WY (梁文艳), Wang K (王珂), Wang JL (王金丽), Ruan QY (阮清鸳). Optimization and evaluation of TTC-dehydrogenase a ssay on a lgal viability determination. Environ Sci & Manage (环境科学与管理), 2009, 34 (5): 10~13
13 Nicole ML, Dong ZM. Microbial nature of the hydrogen-oxidizing agent in hydrogen- treatde soil. Biol Fertil Soil, 2002, 35: 465~469
14 Wei ZX (韦泽秀), Liang YL (梁银丽), Inoue M (井上光弘), Zhou MJ (周茂娟), Huang ML (黄茂林), Gu JF (古建锋), Wu Y (吴燕). Effects of different water and fertilizer supply on cucumber soil nutrient content, enzymeactivity, and microbial diversity. Chin J Appl Ecol (应用生态学报), 2009, 20 (7): 1678~1684

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备注/Memo

备注/Memo:
国家自然科学重点基金项目(No. 30630054)资助 Supported by the National Natural Science Foundation of China (No. 30630054)
更新日期/Last Update: 2010-08-25