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[1]关统伟,赵珂,夏占峰,等.新疆高盐环境土壤放线菌分离培养基比较[J].应用与环境生物学报,2010,16(03):429-431.[doi:10.3724/SP.J.1145.2010.00429]
 GUAN Tongwei,ZHAO Ke,XIA Zhanfeng,et al.Comparison of Isolation Media for Actinobacteria from Different Saline Environments in Xinjiang, China[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):429-431.[doi:10.3724/SP.J.1145.2010.00429]
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新疆高盐环境土壤放线菌分离培养基比较()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年03期
页码:
429-431
栏目:
技术与方法
出版日期:
2010-06-25

文章信息/Info

Title:
Comparison of Isolation Media for Actinobacteria from Different Saline Environments in Xinjiang, China
作者:
关统伟赵珂夏占峰吕玲玲张小平张利莉
(1塔里木大学塔里木盆地生物资源保护利用兵团重点实验室 阿拉尔 843300)
(2四川农业大学资源环境学院 雅安 625000)
(3微生物资源前期开发国家重点实验室 北京 100101)
Author(s):
GUAN Tongwei ZHAO Ke XIA Zhanfeng LÜ Lingling ZHANG Xiaoping ZHANG Lili
(1Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Constuction Corps,
Tarim Univercity, Alar 843300, Xinjiang, China)
(2Faculty of Resource and Environmental Sciences, Sichuan Agricultural University, Ya’an 625000, Sichuan, China)
(3State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China)
关键词:
高盐环境放线菌分离培养基
Keywords:
saline environment actinobacteria isolation medium
分类号:
Q93-335 : Q939.130.8 (245)
DOI:
10.3724/SP.J.1145.2010.00429
文献标志码:
A
摘要:
根据采样点及其样品相关信息结合近几年对高盐环境放线菌的分离经验,设计出一种含有10%~30%复合盐的CMKA培养基,同时以补充10%~30%复合盐的高氏一号琼脂培养基(GA)、ISP 4培养基、淀粉酪素琼脂培养基(SCK)和HV培养基为对照,对采集于新疆阿克陶盐山、罗布泊洼地和阿克苏盐碱地的3份土壤样品利用上述5种培养基进行了分离. 结果表明,CMKA培养基分离效果最好,共分离到7个属的放线菌(Actinopolyspora、Streptomonospora、Nocardiopsis、Saccharomonospora、Brevibacterium、Streptomyces和Amycolatopsis),并且获得1个放线菌新种(TRM F103). ISP 4、GA、HV和SCK培养基分离效果较差,获得的属级分类单元少. 同时,CMKA培养基的出菌率也是最高的,说明比较适合分离高盐环境土壤放线菌,而用于分离普通环境中放线菌的ISP 4、GA、HV和SCK培养基则不适于高盐环境放线菌的分离. 此外,新疆的高盐环境土壤中存在着丰富的放线菌资源,包括一些新类群的存在. 图1 表2 参13
Abstract:
Five media, namely, CMKA, GA, ISP 4, HV and SCK mixed with NaCl, KCl and MgCl2 at concentrations ranging between 10%~30% (w/V), were compared and used to isolate actinobacteria from saline soils in Akesu, Luobopo and Aketao in Xinjiang, China. The results indicated that the CMKA was the best among the five media for isolating actinobacteria. Actinopolyspora, Streptomonospora, Nocardiopsis, Saccharomonospora, Brevibacterium, Streptomyces and Amycolatopsis were isolated by CMKA, including one novel species, named as TRM F103 and belonging to Amycolatopsis. The other four media were found not so good and only four genera were isolated with, namely, Streptomonospora, Actinopolyspora, Nocardiopsis and Streptomyces. So, HV, GA, ISP 4 and SCK were not suitable to isolate actinobacteria from saline environment, but they could be used to isolate actinobacteria from common environment soil. This study also indicated that in the saline soils in this region there were not only abundant actinomycetes, but also some unknown actionobacterial groups existed there. Fig 1, Tab 2, Ref 13

参考文献/References:

1 郑喜玉, 张明刚, 徐昶. 中国盐湖志. 北京: 科学出版社, 2002
2 Guan TW (关统伟), Zhao K (赵珂), Xia ZF (夏占峰), Shun HZ (孙红专), Zhang LL (张利莉). Structure of a soil sample from Yutian Pit in Xinjiang revealed by culture-independent method. Microbiol Bull (微生物学通报), 2009, 36 (4): 515~521
3 Guan TW (关统伟), Wu JY (吴晋元), Zhi XY (职晓阳), Tang SK (唐蜀昆), Li WJ (李文均), Zhang LL (张利莉). Actinobacterial diversity of a sediment sample from Xiaoerkule Lake. Acta Microbiol Sin (微生物学报), 2008, 48 (7): 851~856
4 Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966, 16: 313~340
5 Hayakawa M. Studies on the isolation and distribution of rare actinomycetes in soil. Actionmycetologica, 2008, 22: 12~19
6 Cui XL, Mao PH, Tseng M, Li WJ, Zhang LP, Xu LH, Jiang CL. Streptimonospora salina gen. nov., sp. nov., a new member of family Nocardiopsaceae. Int J Syst Evol Microbiol, 2001, 51: 357~363
7 Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic tree. Mol Biol Evol, 1987, 4: 406~425
8 Felsenstein J. Confidence limits on phylogenies: An approach using the bootstap. Evolution, 1985, 39: 783~791
9 Stackebrandt E, Goebel BM. Taxonomic note: A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol, 1994, 44: 846~849
10 Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol, 1980, 16, 111~120
11 Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol, 1987, 4: 406~425
12 Tang SK (唐蜀昆), Jiang Y (姜怡), Zhi XY (职晓阳), Li WJ (李文均), Xu LH (徐丽华). Isolation methods of halophilic actinomycetes. Microbiol Bull (微生物学通报), 2007, 34 (2): 6199~6200
13 Wu JY, Guan TW, Jiang HC, Zhi XY, Tang SK, Dong HL, Zhang LL, Li WJ. Diversity of actinobacterial community in saline sediments from Yunnan and Xinjiang, China. Extremophiles, 2009, 13: 623~632

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备注/Memo

备注/Memo:
教育部科学技术研究重点项目(No.209145)、国家自然科学基金项目(No. 30660005)、微生物资源前期开发国家重点实验室开放课题(No.SKLMR-20090603)、新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放课题(No. BR0803)和新疆高等学校科研计划创新研究群体基金项目(No. XJEDU2005G07)资助 Supported by the National Natural Science Foundation of China (No. 30660005), the Key Science and Technology Project of Ministry of Education of China (No. 209145), the Opening Project of State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences (No. SKLMR-20090603), the Opening Project of Key Laboratory of Protection and Utilization of Biological Resources in the Tarim Basin of Xinjiang Production & Construction Corps, China (No. BR0803) and the Program of the Higher Education Institution of Xinjiang, China (No. XJEDU2005G07)
更新日期/Last Update: 2010-06-23