|本期目录/Table of Contents|

[1]冷玲,李顺鹏,张迹.甲苯降解菌JB-1的分离、鉴定及其降解特性研究[J].应用与环境生物学报,2010,16(01):118-121.[doi:10.3724/SP.J.1145.2010.00118]
 LENG Ling,ZHANG Ji,LI Shunpeng.Isolation, Identification and Characteristics of A Toluene-Degrading Bacterium JB-1[J].Chinese Journal of Applied & Environmental Biology,2010,16(01):118-121.[doi:10.3724/SP.J.1145.2010.00118]
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甲苯降解菌JB-1的分离、鉴定及其降解特性研究()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年01期
页码:
118-121
栏目:
研究论文
出版日期:
2010-02-25

文章信息/Info

Title:
Isolation, Identification and Characteristics of A Toluene-Degrading Bacterium JB-1
作者:
冷玲李顺鹏张迹
南京农业大学生命科学学院 农业部农业环境微生物工程重点开放实验室 南京 210095
Author(s):
LENG Ling ZHANG Ji LI Shunpeng
(Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China)
关键词:
甲苯生物降解假单胞菌属甲苯双加氧酶邻苯二酚23双加氧酶代谢途径
Keywords:
toluene biodegradation Pseudomonas sp. toluene dioxygenase catechol 23-dioxygenase metabolic pathway
分类号:
X172
DOI:
10.3724/SP.J.1145.2010.00118
文献标志码:
A
摘要:
从南京石化废水处理池的活性污泥中分离到1株能以甲苯为唯一碳源生长的细菌,命名为JB-1. 根据其生理生化特征和16S rDNA(GenBank Accession No. EU869278)序列相似性分析,将该菌株鉴定为假单胞菌属(Pseudomonas sp.). 该菌株在18 h内能完全降解346.4 mg/L的甲苯. 降解甲苯的最适温度为30 ℃,pH为7.0,降解速率与初始接种量呈正相关. 甲苯降解途径是通过甲苯双加氧酶形成顺甲苯二氢二醇,再经邻苯二酚2,3双加氧酶开环降解. 图2 参22
Abstract:
A bacterial strain JB-1 capable of utilizing toluene as sole carbon source was isolated from active sludge collected from Nanjing Petro-chemical Co., Ltd. It was preliminarily identified as Pseudomonas sp. according to its physiological and biochemical properties and the similarity analysis of its 16S rRNA gene sequence (GenBank Accession No. EF869278). Strain JB-1 could degrade 346.4 mg/L toluene within 18 h. The optimal pH and temperature for the degradation were 7.0 and 30 ℃, respectively. The degrading rate showed a positive correlation with the initial inoculum size. The biodegradation pathway of toluene has formed cis-toluene dihydrodiol by toluene dioxygenase. Fig 2, Ref 22

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备注/Memo

备注/Memo:
国家自然科技资源平台项目(No. 2005DKA21201-11)
更新日期/Last Update: 2010-02-09