|本期目录/Table of Contents|

[1]段学军** 黄春晓.重金属镉对水田土壤微生物基因多样性的影响[J].应用与环境生物学报,2008,14(04):510-513.
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重金属镉对水田土壤微生物基因多样性的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
14卷
期数:
2008年04期
页码:
510-513
栏目:
研究论文
出版日期:
2008-05-15

文章信息/Info

作者:
段学军** 黄春晓
(中原工学院能源与环境学院 郑州 450007)
关键词:
DGGE 稻田土壤微生物基因多样性CLC S154.3 : X171
文献标志码:
A
摘要:
采用16S rDNA DGGE 分析技术,探讨了不同处理时间、不同浓度镉胁迫条件下水田土壤微生物种群的基因多样性. 研究发现,随着处理时间的不同,各处理间的土壤微生物基因多样性差异存在明显不同,培养初期镉对土壤微生物种群毒害作用非常明显,土壤微生物基因多样性下降,但培养后期不同处理之间DNA 基因型差异很小,丰度差异较大. 在土壤培养初期、含镉3~10 mg/kg 的培养基上出现一条新增条带,为对照及低镉浓度土壤所没有,镉形态稳定后会消失,说明土壤中可能存在适宜镉浓度下良好生长的微生物种,经序列测定及与网上比对分析,与一株Uncultured bacterium sp. 具有96% 的相似率,初步确定为一不可培养细菌种,以其作为淹水条件下镉污染初期的报告基因有一定的可行性. 图6 参18

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更新日期/Last Update: 2009-01-16