|本期目录/Table of Contents|

[1]王春明,李大平,刘世贵.4株多环芳烃降解菌的分离及鉴定[J].应用与环境生物学报,2007,13(04):546-550.
 WANG Chunming,et al..Screening and Identification of Four PAHsbiodegrading Strains[J].Chinese Journal of Applied & Environmental Biology,2007,13(04):546-550.
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4株多环芳烃降解菌的分离及鉴定()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
13卷
期数:
2007年04期
页码:
546-550
栏目:
综述
出版日期:
2007-08-25

文章信息/Info

Title:
Screening and Identification of Four PAHsbiodegrading Strains
作者:
王春明李大平刘世贵
(1四川大学生命科学学院生物资源与生态环境教育部重点实验室 成都 610064) (2中国科学院成都生物研究所 成都 610041)
Author(s):
WANG Chunminget al.
(1Key Laboratory of Bioresources and Ecoenvironment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China) (2Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
关键词:
关键词 多环芳烃 16S rDNA生物降解 序列同源性
Keywords:
Keywords polycyclic aromatic hydrocarbons (PAHs) 16S rDNA biodegradation sequence similarity
摘要:
摘要 以多环芳烃(PAHs)为筛选培养基,从石油污染土壤和石油废水中筛选到4株能够降解PAHs的高效微生物菌株,分别定名为328、 NF、 EW和EY;并对其进行了形态学观察、生理生化指标测定及分子生物学鉴定.16S rDNA序列分析显示,这4株细菌分别有581、 582、 1 209和1 230个碱基,与Microbacterium、Cellulosimicrobium、 Chelatococcus和Sphingopyxis等4个属有较高的序列同源性,分别为100%、 97.8%、 98.2%和99.0%.结合表型特征和16S rDNA序列分析,用DNAMAN构建系统发育树,并用Bootstraping法对其评价,初步将328、 NF、 EW和EY这4株PAHs降解菌分别归属于Microbacterium sp.、 Cellulosimicrobium sp.、 Chelatococcus sp.和Sphingopyxis sp..图3 表1 参22
Abstract:
Abstract Microbes play an important role in bioremediation technologies, and it is a basic study to find out effectively functional strains for environmental bioremediation. In this paper four PAHsbiodegrading strains were screened with a selective medium of PAHs from oily wastewater and oilcontaminated soil. The four strains were identified by their morphological, physiological and biochemical characteristics and gene analyses, and they were named as 328, NF, EW and EY. The 16S rDNA of the strains was amplified by PCR, indicating that they possessed 581, 582, 1209, 1 230 bp, respectively, which were compared with those available in the GenBank databases. The results showed that the 16S rDNA sequence of 328 strain was mostly similar to that of Microbacterium esteraromaticum, NF similar to Cellulosimicrobium cellulans, EW to Chelatococcus asacharovorans and EY to Sphingopyxis terrae, and their sequence similarities were 100%, 978%, 982% and 990%, respectively. The phylogenetic trees of the four strains were derived with DNAMAN and analyzed with Bootstraping. The results demonstrate that the 328, NF, EW and EY strains belonged to Microbacterium sp., Cellulosimicrobium sp., Chelatococcus sp. and Sphingopyxis sp., respectively. Fig 3, Tab 1, Ref 22

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更新日期/Last Update: 2007-08-29