|本期目录/Table of Contents|

[1]胡忠,吴奕瑞,徐艳,等.海洋苯酚降解菌Candida sp. P5的分离鉴定及其降解特性[J].应用与环境生物学报,2007,13(02):243-247.
 HU Zhong,et al..Screening of a Marine Phenoldegrading Yeast Candida sp. P5 and its Biodegradation Characteristics[J].Chinese Journal of Applied & Environmental Biology,2007,13(02):243-247.
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海洋苯酚降解菌Candida sp. P5的分离鉴定及其降解特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
13卷
期数:
2007年02期
页码:
243-247
栏目:
综述
出版日期:
2007-04-25

文章信息/Info

Title:
Screening of a Marine Phenoldegrading Yeast Candida sp. P5 and its Biodegradation Characteristics
作者:
胡忠吴奕瑞徐艳黄通旺郑天凌
(汕头大学生物学系广东汕头515063; 1厦门大学生命科学学院福建厦门361005)
Author(s):
HU Zhonget al.
(Department of Biology, Shantou University, Shantou 515063, Guangdong, China) (1School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China)
关键词:
关键词 苯酚生物降解假丝酵母菌P5
Keywords:
Keywords phenol biodegradation Candida sp. P5
摘要:
摘要 从海洋沉积物中分离、筛选到一株能以苯酚作为唯一碳源和能源的酵母菌P5.根据菌落特征、菌体形态、生理生化特性和18S rDNA序列分析,确定菌株P5为假丝酵母菌属(Candida sp.).该菌株最适宜生长和降解苯酚的条件为:温度25 ℃, pH 6.0~7.0,摇床转速100 r/min,需氧;菌株P5能在较高浓度的苯酚条件下生长,在72 h内可以降解95%以上的苯酚.对苯酚代谢途径和相关酶的研究发现,菌株P5主要在邻苯二酚1,2双加氧酶作用下通过邻位途径进行苯酚代谢.图7表2参24
Abstract:
Abstract Strain P5, capable of degrading phenol, was isolated from marine sediment and could use phenol as the sole carbon source for growth. It was identified as Candida sp. by its morphologic and physiological characteristics, especially by the analysis of 18S rDNA sequences. Its optimal conditions for growth and degradation were 25 ℃, pH 6.0~7.0, 100 r/min of shaking rate and oxygen required. Strain P5 could grow at a relative higher concentration of phenol and degrade over 95% of phenol within 72 h. The metabolism of phenol was catalyzed by catechol 1,2dioxygenase of Candida sp. P5 through the meta pathway. Fig 7, Tab 2, Ref 24

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更新日期/Last Update: 2007-04-24