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[1]王明秀,栾威 马欣荣 陶 向** 赵 云**.少根紫萍淀粉合成关键基因对寡营养胁迫的响应*[J].应用与环境生物学报,2018,24(06):1-14.[doi:10.19675/j.cnki.1006-687x.2018.04021]
 WANG Mingxiu,LUAN Wei,MA Xinrong,et al.Research of starch biosynthesis related genes and the responses to nutrition starvation in Landoltia punctata *[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1-14.[doi:10.19675/j.cnki.1006-687x.2018.04021]
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少根紫萍淀粉合成关键基因对寡营养胁迫的响应*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1-14
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
Research of starch biosynthesis related genes and the responses to nutrition starvation in Landoltia punctata *
文章编号:
201804021
作者:
王明秀1 2 栾威2 马欣荣2 陶 向2** 赵 云1**
1四川大学生命科学学院 成都 6 10041
2中国科学院成都生物研究所 成都 610041
Author(s):

1College of Life Sciences, Sichuan University, Chengdu 610041, China ?
2Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
关键词:
少根紫萍寡营养淀粉合成基因转录组荧光定量PCR
Keywords:
Landoltia punctata nutrient starvation starch biosynthesis gene transcriptome qRT-PCR
DOI:
10.19675/j.cnki.1006-687x.2018.04021
摘要:
浮萍是一种形态高度退化的开花植物,环境适应性广,生长条件适宜时可以接近指数增长的速度在16-48小时内实现生物量的翻倍。以寡营养处理,少根紫萍(Landoltia punctata)淀粉含量可在7天内提高到45%以上(干重)。但目前少根紫萍淀粉代谢相关基因的组成与表达研究较少。本研究通过深入分析转录组数据,分别挖掘到ADP葡萄糖焦磷酸化酶编码基因(LpAGP)5个、颗粒结合型淀粉合酶基因(LpGBSS)2个、可溶性淀粉合酶基因(LpSSS)2个、淀粉分支酶基因(LpSBE)5个、异淀粉酶基因7个(LpISA)、普鲁兰酶基因(LpPUL)1个。转录组定量结果表明LpAGPS1、LpAGPL2、LpAGPL3、LpGBSSI、LpGBSSII、LpSBEI-1、 LpISA3 和LpPUL1的表达量均因寡营养处理而上调。通过qRT-PCR检测LpAGPs等16个淀粉合成关键基因的表达量,发现绝大部分淀粉合成相关基因表达量上调,而参与淀粉降解途径相关基因(如α-淀粉酶和β-淀粉酶编码基因)的表达量因寡营养处理而下调。这种不同的调节方式促使少根紫萍淀粉合成“开源节流”,淀粉快速积累。该研究为进一步研究少根紫萍淀粉合成关键基因功能及淀粉积累机制奠定了基础。(图3 表2 参33)
Abstract:
Objectives: Landoltia punctata can accumulate high starch content to >45% (dry weight) in 7 days treated by nutrient starvation. This study aimed to reveal the genetic makeup of starch metabolism related genes and expression pattern of these genes in response to starvation .Methods: Starvation response transcriptome of L. punctata was carefully analyzed, and used for the identification and quantification of starch metabolism related genes. qRT-PCR was performed to verify the quantification results. Results: The results demonstrated that there were five LpAGPs, two LpGBSSs, two LpSSSs, five LpSBEs, seven LpISAs and one LpPUL. RNA-Seq quantification results showed that LpAGPS1, LpAGPL2, LpAGPL3, LpGBSSI, LpGBSSII, LpSBEI-1, LpISA3 and LpPUL1 were all up-regulated by nutrient starvation. qRT-PCR results of 16 starch metabolism key genes verified that most of them were up-regulated by starvation, while the expression of α-amylase and β-amylase were down-regulated. Conclusions: The opposite expression change patterns of starch biosynthesis and degradation related key genes resulted in the accumulation of starch. This study pave d the way for the further function and mechanism studies of starch metabolism involved genes in L. punctata.

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备注/Memo

备注/Memo:
收稿日期: 2018-04-18 接受日期: 2018-05-02
*国家自然科学基金项目(31400218)资助?
**通讯作者(E-mail: zhaoyun@scu.edu.cn, taoxiang@cib.ac.cn)
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更新日期/Last Update: 2018-06-05