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[1]孙 娟 李 东** 郑 涛** 刘晓风 陈 琳 何明阳.微量元素对蔬菜废弃物连续厌氧消化系统微生物群落结构的影响*[J].应用与环境生物学报,2018,24(06):1-10.[doi:10.19675/j.cnki.1006-687x.2018.03035]
 SUN Juan,LI Dong**,ZHENG Tao**,et al.Effects of trace elements on microbial community structure in continuous anaerobic digestion of vegetable waste *[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1-10.[doi:10.19675/j.cnki.1006-687x.2018.03035]
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微量元素对蔬菜废弃物连续厌氧消化系统微生物群落结构的影响*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1-10
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
Effects of trace elements on microbial community structure in continuous anaerobic digestion of vegetable waste *
文章编号:
201803035
作者:
孙 娟1 李 东2** 郑 涛1** 刘晓风1 陈 琳1 何明阳1
1常州大学城乡矿山研究院常州213100
2中国科学院成都生物研究所成都 610041
Author(s):
SUN Juan1 LI Dong2** ZHENG Tao1** CHEN Lin1 & HE Mingyang1
1Institute of Urban and Rural Mines, Changzhou University, Changzhou 213164, China;
2Chengdu Institute of Biology, Chinese Academy of Sciences,Chengdu610041, China
关键词:
蔬菜废物物厌氧消化微量元素微生物群落结构
Keywords:
vegetable waste anaerobic digestion trace element microbial community structure
DOI:
10.19675/j.cnki.1006-687x.2018.03035
摘要:
以蔬菜废弃物为原料的厌氧消化过程产甲烷能力下降时,通过添加微量元素使其恢复稳定状态,研究微量元素对厌氧消化系统微生物结构的影响对优化系统性能具有重要意义。本研究采用70 L厌氧发酵罐,有效体积59.5 L,在中温35 ℃条件下进行蔬菜废弃物厌氧消化的连续冲击负荷试验,根据CH4含量变化规律,及时添加微量元素(Fe, Co, Ni)促进厌氧消化过程。样品采用16S rRNA基因扩增和MiSeq测序技术分析微生物群落的结构。结果表明,微量元素对细菌群落的影响主要作用于拟杆菌菌门、厚壁菌门及螺旋菌门。在属水平上,第一次微量元素的添加诱导了拟杆菌门中的vadinBC27 wastewater-sludge的增加,相对丰度从54.1%升至68%,降低了厚壁菌门中Erysipelotrichaceae UGG-004 以及螺旋菌门中Sphaerochaeta。第二次微量元素的添加,主要降低了螺旋菌门中的Sphaerochaeta,相对丰度从11.4%到4.4%,以及诱导拟杆菌门中Bacteroides的产生,提高了原料利用率,降低了酸化的抑制作用。微量元素对蔬菜废弃物厌氧消化过程中产甲烷菌群落的影响主要在甲基营养型Candidatus Methanoplasma、甲烷鬃菌属为主导的乙酸营养型。当挥发性脂肪酸含量较高时,Candidatus Methanoplasma占主导地位,微量元素添加后则会诱导甲烷鬃菌为主导的乙酸营养型甲烷菌的产生,相对丰度从2.3%增至80%促进挥发性脂肪酸的消耗转化。因此,微量元素的添加对于微生物群落结构的改变显著,促进厌氧消化过程水解酸化与甲烷化的平衡,从而稳定运行。(图5表2参27)
Abstract:
To optimize performance of continuous anaerobic digestion of vegetable waste, the relationship between trace elements and microbial community structure was explored. In this study, continuous anaerobic digestion (AD) of vegetable waste (VW) was carried out in the 70 L anaerobic reac tors under mesophilic conditions (35 ℃) and the trace elements supplementation was based on the CH 4 contentin order to stabilize the process under high OLR for long run-length. The microbial community structure was obtained by using MiSeq sequencing of 16S rRNA gene amplicons. For the bacterial communities, the addition of trace elements had significant effects on Bacteroidetes、Spirochaetae and Firmicutes. At the genus level, the first trace elements addition induced vadinBC27 waste water-sludge to increase to 68% from 54.1% , and reduced Erysipelotrichaceae UGG-004 and Sphaerochaeta. The first trace elements addition induced Bacteroides to increase and reduced the Sphaerochaeta from 11.4% to 4.4% to improve the utilization of raw materials and reduced the inhibition of acidification. For the methanogen communities, the addition of trace elements had significant effects on Candidatus Methanoplasma which was methantrophic-utilization methanogens and acetic-utilization methanogens with Methanosaeta dominated. Candidatus Methanoplasma dominated under high volatile fatty acids ( VFA), and Methanosaeta dominated from 2.3% to 80% after the addition of trace elements to promote the consumption and conversion of VFA . Therefore, the trace elements can promote the balance of hydrolysis and acidification and methanation in anaerobic digestion of vegetable waste, thus stabilizing the operation.

相似文献/References:

[1]沈东升,徐向阳,冯孝善.进水PCP和CODCr质量浓度对厌氧反应器运行效能的影响[J].应用与环境生物学报,1997,3(04):375.
 Shen Dongsheng,Xu Xiangyang,Feng Xiaoshan.EFFECT OF PCP & CODcr MASS CONCENTRATIONS IN INFLUENT ON OPERATIONAL BEHAVIORS OF ANAEROBIC REACTORS[J].Chinese Journal of Applied & Environmental Biology,1997,3(06):375.
[2]向元英,杨暖,孙霞,等.单室微生物电解池强化混合脂肪酸产甲烷*[J].应用与环境生物学报,2016,22(05):872.[doi:10.3724/SP.J.1145.2015.12009]
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备注/Memo

备注/Memo:
收稿日期: 2018-03-23 接受日期: 2018-04-25
*国家自然科学基金面上项目(21476222)、中国科学院战略生物资源服务网络计划生物资源衍生库项目(ZSYS-009)、中国科学院青年创新促进会资助项目(2017423)资助?
**通讯作者(E-mail:lidong@cib.ac.cn,hellozheng@gmail.com)
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更新日期/Last Update: 2018-06-05