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[1]苟艳 谢天 蒲莉 王刚刚**.解淀粉芽孢杆菌MY001菌株对几丁质的降解及对芦笋茎枯病菌的拮抗作用*[J].应用与环境生物学报,2018,24(06):1-10.[doi:10.19675/j.cnki.1006-687x.2018.03024]
 GOU Yan,XIE Tian,PU Li,et al.The Degradation of Chitin and Antagonism on Phomopsis aspasagi by Bacillus amyloliquefaciens MY001 *[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1-10.[doi:10.19675/j.cnki.1006-687x.2018.03024]
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解淀粉芽孢杆菌MY001菌株对几丁质的降解及对芦笋茎枯病菌的拮抗作用*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1-10
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
The Degradation of Chitin and Antagonism on Phomopsis aspasagi by Bacillus amyloliquefaciens MY001 *
文章编号:
201803024
作者:
苟艳123 谢天12 蒲莉12 王刚刚12**
1中国科学院成都生物研究所中国科学院环境与应用微生物重点实验室 成都 610041
2中国科学院成都生物研究所环境微生物四川省重点实验室 成都 610041
3中国科学院大学 北京 100049
Author(s):
GOU Yan123 XIE Tian12 PU Li12 WANG Ganggang12 **
1Key Laboratory of Environmental and Applied Microbiology of Chinese Academy of Sciences, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China2Key Laboratory of Environmental Microbiology of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China3University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
解淀粉芽孢杆菌壳聚糖酶几丁质降解天门冬拟茎点霉拮抗作用
Keywords:
Bacillus amyloliquefaciens chitosanase chitin degradation Phomopsis aspasagi antagonistic effect
DOI:
10.19675/j.cnki.1006-687x.2018.03024
摘要:
几丁质每年自然总产量高达100亿吨,目前,由于工业上缺乏清洁高效的几丁质降解工艺,而使几丁质资源的利用受到严重制约。作者以壳聚糖为唯一碳源从土壤中分离筛选出一株具有产壳聚糖酶能力的菌株MY001,发酵上清酶活力约为2.7 U/mg,利用16S rDNA、 gyrA及gyrB基因测序的方法将该菌株鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。通过分析MY001菌株在不同培养基中的生长曲线特点,结合MY001菌株降解后的几丁质的扫描电镜观察结果,发现该菌株可以直接利用几丁质。另外,发现该菌株对芦笋茎枯病菌[Phomopsis aspasagi ( Sacc.) Bubak]具有拮抗作用。当胶体几丁质和MY001菌株同时存在时,对芦笋茎枯病菌的抑制更加明显。本文研究结果表明,解淀粉芽孢杆菌MY001菌株具有降解几丁质和拮抗芦笋茎枯病菌的功能,而且添加几丁质具有拮抗增效作用,因此,该菌株有望用于几丁质资源清洁利用和农作物真菌病害生物防治。(图6表1参40)
Abstract:
Objectives: Chitin, as a natural polymer, is generated about 10 billion tons annually. However, the utilization of chitin was hindered because no clean and effective degradation process was established.Methods: The bacterial strains with chitosanase-producing ability were isolated from soil and identified by 16S rDNA, gyrA, gyrB gene sequence analysis. In addition, the degradation of insoluble chitin and antifungal capability of the bacterial strains were investigated. Results: One selected isolate was identified as Bacillus amyloliquefaciens strain MY001. It has an antagonistic effect on Phomopsis aspasagi (Sacc.) Bubak and can degrade insoluble chitin. The combination of chitin/chitosan and MY001 strain could enhance the inhibition on the growth of P. aspasagi. Conclusions: The strain B. amyloliquefaciens MY001 can directly degrade chitin. The combination of MY001 strain with chitin shows more effective inhibition on P. aspasagi. These results show light on the direct utilization of chitin against fungal diseases of crops.

相似文献/References:

[1]费忠,李恒,龚劲松,等.基于宏基因组学壳聚糖酶挖掘研究[J].应用与环境生物学报,2014,20(04):597.[doi:10.3724/SP.J.1145.2014.01039]
 FEI Zhong,LI Heng,GONG Jinsong,et al.Studies of chitosanase mining based on metagenomic technology[J].Chinese Journal of Applied & Environmental Biology,2014,20(06):597.[doi:10.3724/SP.J.1145.2014.01039]

备注/Memo

备注/Memo:
收稿日期: 2018-03-16 接受日期: 2018-04-03
*中国科学院百人计划项目、中国科学院科技服务网络计划项目(KFJ-SW-STS-143-7)资助?
**通讯作者(E-mail:wanggg@cib.ac.cn)
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更新日期/Last Update: 2018-04-16