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[1]郭玉琼 王 仲 朱 晨 赵姗姗 张舒婷 常笑君 李小桢 傅海峰 林玉玲 赖钟雄**.茶树CSD1基因及其启动子克隆与低温胁迫下的表达分析*[J].应用与环境生物学报,2018,24(05):1-15.[doi:10.19675/j.cnki.1006-687x.2018.02021]
 GUO Yuqiong,WANG Zhong,ZHU Chen,et al.Cloning and expression analysis of copper/zinc-superoxide dismutase 1 gene and its promotor under low temperature stress in Camellia sinensis*[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1-15.[doi:10.19675/j.cnki.1006-687x.2018.02021]
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茶树CSD1基因及其启动子克隆与低温胁迫下的表达分析*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年05期
页码:
1-15
栏目:
研究论文
出版日期:
2018-10-25

文章信息/Info

Title:
Cloning and expression analysis of copper/zinc-superoxide dismutase 1 gene and its promotor under low temperature stress in Camellia sinensis*
文章编号:
201802021
作者:
郭玉琼1 王 仲13 朱 晨1 赵姗姗1 张舒婷12 常笑君12 李小桢1 傅海峰1 林玉玲12 赖钟雄12**
1福建农林大学园艺学院 福州 350002
2福建农林大学园艺植物生物工程研究所 福州 350002
3贺州学院食品与生物工程学院 贺州 542899
Author(s):
GUO Yuqiong1 WANG Zhong12 ZHU Chen1 ZHAO Shanshan1 ZHANG Shuting12 CHANG Xiaojun12 LI Xiaozhen1 FU Haifeng1 LIN Yuling12 & LAI Zhongxiong12**
1College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China?
2Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China?
3College of Food and Biological Engineering, Hezhou University, Hezhou 542899, China
关键词:
茶树CSD1基因低温胁迫表达分析
Keywords:
Camellia sinensis Cu/Zn-superoxide dismutase gene low-temperature stress expression analysis
DOI:
10.19675/j.cnki.1006-687x.2018.02021
摘要:
低温寒害严重影响茶树生长发育状况及成品茶品质,Copper/zinc-superoxide dismutase (CSD)基因在植物抗胁迫响应中起关键作用。为了解CsCSD基因响应低温胁迫的作用机制,以铁观音茶树叶片为供试材料,采用同源克隆结合RACE方法及染色体步移法,获得茶树CSD1基因的cDNA序列(GenBank 登录号:KR078346)、gDNA序列及其启动子序列,并对其进行了生物学信息学分析,同时对低温胁迫处理下CsCSD1基因的表达模式也进行了分析。结果表明,茶树CsCSD1基因cDNA全长860 bp,完整的开放阅读框(ORF)长度为459 bp,共编码152个氨基酸;gDNA基因结构分析发现CsCSD1基因由6个外显子和5个内含子构成;CpG岛预测发现启动子区域内存在1个CpG岛,CpG长度为219 bp,GC含量为50.3%;CsCSD1启动子上还存在大量的顺式作用元件,包括光响应元件、激素响应元件、胁迫响应元件和其他响应元件;在低温胁迫下CsCSD1基因的表达模式显示,在低温胁迫前期,CsCSD1基因表达上调;随后CsCSD1基因的表达量不断下降。CsCSD1基因能够响应低温胁迫并在胁迫的不同时期发挥不同的作用,推测与低温胁迫密切相关。(图7 表3 参50)
Abstract:
Objectives: Low temperature stress severely affects the growth and quality of tea. Copper/zinc-superoxide dismutase (CSD) genes play crucial roles in the plant response to stresses. The objective of this study is to explore the adversity response mechanism of CsCSD gene under low temperature stress.Methods: The leaves of Camellia sinensis cv. Tieguanyin were used as materials. The full-length cDNA (GenBank accession number: KR078346), gDNA and promoter sequence of CsCSD1 gene were cloned by the homology-based cloning combined with RACE technique and chromosome walking. Moreover, the characteristic of bioinformatics and the expression patterns of CsCSD1 gene under low temperature stress were investigated. Results: The results showed that the full-length cDNA of CsCSD1 gene was 860 bp, containing a 459 bp open reading frame (ORF) which encodes a protein of 152 amino acids. The gDNA structural analysis found that the CsCSD1 gene was composed of 6 exons and 5 introns. And the CpG islands prediction found that there was only a CpG island in the promoter region. The length of CpG inland was 219 bp and the percentage of GC content was 50.3%. The CsCSD1 promoter also contained a large number of cis-acting elements, including light response elements, hormone response elements, stress response elements, and other response elements. The expression analysis revealed that the expression of CsCSD1 was up-regulated at the early stage of low temperature stress, and then the expression decreased continuously. Conclusions: The CsCSD1 gene can respond to low temperature stress and play a different role in different periods of low temperature stress, which was presumed to be related to low temperature stress.

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备注/Memo

备注/Memo:
收稿日期 Received: 2018-02-10 接受日期 Accepted: 2018-03-21
*福建省重大科技专项(2015NZ0002-1)、福建省教育厅资助项目(JA15143)、福建农林大学科技创新专项基金项目(CXZX2017164)资助?
**通讯作者(E-mail:Laizx01@163.com)
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更新日期/Last Update: 2018-04-16