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[1]曾 丹,钟 娟 谭 红 周金燕,**.基于卤虫致死试验分离淡色生赤壳菌YLZ42杀虫活性代谢产物*[J].应用与环境生物学报,2018,24(05):1-9.[doi:10.3724/SP.J.1145.2018.02001]
 ZENG Dan,ZHONG Juan,et al.Isolation of insecticidal metabolite from Bionectria ochroleuca YLZ42 based on the brine shrimp lethality test *[J].Chinese Journal of Applied & Environmental Biology,2018,24(05):1-9.[doi:10.3724/SP.J.1145.2018.02001]
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基于卤虫致死试验分离淡色生赤壳菌YLZ42杀虫活性代谢产物*()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年05期
页码:
1-9
栏目:
研究论文
出版日期:
2018-10-25

文章信息/Info

Title:
Isolation of insecticidal metabolite from Bionectria ochroleuca YLZ42 based on the brine shrimp lethality test *
文章编号:
201802001
作者:
曾 丹1 3 钟 娟1 谭 红1 周金燕1 2 **
1中国科学院成都生物研究所/环境与应用微生物重点实验室 成都 610041
?2四川省环境微生物重点实验室 成都 610041?
?3中国科学院大学 北京 100039
Author(s):
ZENG Dan1 3 ZHONG Juan1 TAN Hong1 ZHOU Jinyan1 2**
1 Key Laboratory of Environmental and Applied Microbiology/Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China?
2 Environmental Microbiology Key Laboratory of Sichu an ProvinceChengdu 610041, China?
3 University of Chinese Academy of Sciences, Beijing 100039, China
关键词:
淡色生赤壳菌卤虫致死试验杀虫活性稳定性
Keywords:
Bionectria ochroleuca brine shrimp lethality test insecticidal activity stability
DOI:
10.3724/SP.J.1145.2018.02001
摘要:
淡色生赤壳菌具有优良的生防潜力,本实验室从广西玉林土壤中分离到一株对小菜蛾等多种农业害虫具有毒杀作用的淡色生赤壳菌YLZ42。对该菌杀虫活性化合物进行分离和稳定性研究,可为其开发应用提供理论指导。本研究采用杀虫活性追踪的方法,用改进后的卤虫致死试验(brine shrimp lethality test,BSLT)进行活性评价,通过硅胶柱层析、萃取、薄层制备、液相制备的手段进行分离纯化,并探索了活性化合物的生物活性和稳定性。结果显示:本研究分离到了杀虫活性化合物纯品,高分辨率质谱显示该活性化合物相对分子质量为788,其对卤虫24 h的LC50为86.89 mg/L 。该化合物在温度低于30 ℃,pH在6-8之间,以及光照和紫外光照条件下,活性稳定。研究表明,该杀虫活性化合物的氢谱与已报道过的分子量为788的化合物均不相同,有望开发为新的微生物源杀虫剂。(图8 表2 参27)
Abstract:
Objectives: Bionectria ochroleuca is potential ly useful for bio-control. B. ochroleuca YLZ42 isolated from soil in Yulin of Guangxi Zhuang Autonomous Region was proved to be able to kill various a gricultural pests, such as the diamondback moth, Plutella xylostella. This paper was aimed to isolate i nsecticidal metabolite from the fermentation broth of B. ochroleuca YLZ42 and study its biological activity and stability.Methods: The isolation of insecticidal metabolite was based on a bioassay-guided method. In consequence, optimized brine shrimp lethality test (BSLT) was chose n to evaluate insecticidal ability. Silica gel column chromatography, extraction, preparative TLC and reverse-phase HPLC were conducted to isolate the insecticidal metabolite. After that, the biological activity and stability of this i nsecticidal metabolite were studied. Furthermore, HR-ESI-MS and 1H-NMR were used to analyze the metabolite.Results: A pure metabolite with insecticidal activity was obtained. The LD 50 against b rine shrimp of this metabolite is 86.89 mg/L at 24 h post-treatment time point. In addition, t he stability of this insecticidal metabolite indicated that insecticidal activity was stable while the temperature is below 30 ℃ and the pH values rang e from 6 to 8. Besides, the metabolite is not sensitive to UV-visible light. Molecular w eight of this compound determined with high-resolution mass spectrometry is 788. Conclusions: The 1H-NMR spectrum of this insecticidal metabolite was different from any other reported compound with the same molecular weight. The insecticidal metabolite has the potential to be developed as a new microbial pesticide.

备注/Memo

备注/Memo:
收稿日期: 2018-02-01 接受日期: 2018-03-12
*四川省科技支撑计划(2016NZ0032)资助?
**通讯作者(E-mail: zhoujy@cib.ac.cn)
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更新日期/Last Update: 2018-03-19