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[1]唐海,姚银安,张国燕,等.异源表达胡杨PeBAK1;1基因增强烟草对Pst DC3000的抗性[J].应用与环境生物学报,2018,24(02):335-343.[doi: 10.19675/j.cnki.1006-687x.2017.10018]
 TANG Hai,YAO Yinan,ZHANG Guoyan,et al.Heterologous overexpression of Populus euphratica BAK1;1 gene enhanced resistance to Pst DC3000 in tobacco[J].Chinese Journal of Applied & Environmental Biology,2018,24(02):335-343.[doi: 10.19675/j.cnki.1006-687x.2017.10018]
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异源表达胡杨PeBAK1;1基因增强烟草对Pst DC3000的抗性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年02期
页码:
335-343
栏目:
研究论文
出版日期:
2018-04-25

文章信息/Info

Title:
Heterologous overexpression of Populus euphratica BAK1;1 gene enhanced resistance to Pst DC3000 in tobacco
作者:
唐海姚银安张国燕李星丁红霞吴英青高永峰
西南科技大学生命科学与工程学院 绵阳 621010
Author(s):
TANG Hai YAO Yin′an ZHANG Guoyan LI Xing DING Hongxia WU Yingqing GAO Yongfeng
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
关键词:
PeBAK11胡杨烟草抗病相关基因Pst DC3000
Keywords:
PeBAK11 Populus euphratica tobacco pathogenesis-related genes Pst DC3000
分类号:
S5.034 : Q786
DOI:
10.19675/j.cnki.1006-687x.2017.10018
摘要:
BAK1(BRI1-associated receptor kinase 1)是一个富亮氨酸重复序列(LRR)的膜受体蛋白激酶,除参与植物油菜素内酯信号的转导外,还可以结合其他的LRR-RLKs蛋白来启动植物的先天免疫反应. 为明确胡杨PeBAK1;1基因在烟草中抗Pst DC3000(丁香假单胞杆菌种番茄致病变种)的功能及其在植物抗病中的调控方式,克隆胡杨PeBAK1;1基因cDNA序列构建PeBAK1;1基因的过量表达载体pBI121-35S::PeBAK1;1,并利用农杆菌介导法将其转化野生型烟草,筛选获得PeBAK1;1基因过表达的转基因烟草植株. 生物信息学分析结果表明胡杨PeBAK1;1蛋白具有植物SERK家族的全部结构特征,系统进化树分析表明胡杨PeBAK1;1与毛果杨PtBAK1的序列同源性最高;组织表达分析表明PeBAK1;1基因主要在根和叶中表达;Pst DC3000侵染试验结果显示,野生型烟草植株接种Pst DC3000后表现出明显的感病症状,而PeBAK1;1基因过表达的转基因烟草植株表现出明显的抗病症状;Real-time PCR及quantitative real-time-PCR分析结果表明,与野生型相比,转基因烟草中抗病防御相关基因PR1、PR3、PR4和PR5的表达量显著升高,且参与植物生长发育与先天防卫反应的基因BIR1和BON1的表达量也明显升高. 上述结果表明,异源表达胡杨PeBAK1;1基因在烟草抗Pst DC3000过程中起正调控作用,能够增强植物的抗病能力. (图6 表1 参31)
Abstract:
BRI1-ASSOCIATED RECEPTORKINASE1 (BAK1), a leucine-rich repeat (LRR) receptor protein kinase, plays a significant role in brassinosteroid (BR) signaling. Furthermore, it combines with other LRR-RLKs protein to initiate immune response in plants. The objective of this study was to (1) investigate the function of the Populus euphratica BAK1;1 gene in the resistance of transgenic tobacco to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and (2) discuss the regulation pathway of PeBAK1;1 involved in the resistance to plant pathogen. We cloned the cDNA sequence of the P. euphratica PeBAK1;1 gene, constructed the pBI121-35S::PeBAK1;1 over-expression vector, and then transformed it into wild-type tobacco by Agrobacterium-mediated transformation to obtain PeBAK1;1-overexpressing transgenic tobacco plants. The bioinformatic analysis showed that the PeBAK1;1 protein contained all the structural features of the plant SERK family. The phylogenetic tree showed that PeBAK1;1 has the highest sequence homology with PtBAK1. The gene expression profile results indicated that the expression of PeBAK1;1 in the root was higher than that in the leaf and stem. The wild-type tobacco plants showed an obvious susceptibility to Pst DC3000, whereas the transgenic plants exhibited enhanced resistance to Pst DC3000. Compared with that of the wild-type (WT), the real-time PCR and quantitative real-time PCR analysis revealed that the expression of pathogenesis-related genes (including PR1, PR3, PR4, and PR5), BAK1-interacting receptor kinase 1 gene, and BONZAI1 gene was upregulated in 35S::PeBAK1;1 transgenic tobacco plants. In conclusion, the PeBAK1;1 gene plays a positive regulatory role in 35S::PeBAK1;1 transgenic tobacco against Pst DC3000, which can enhance the resistance of plants to pathogen.

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更新日期/Last Update: 2018-04-25