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[1]李志丹,方扬,靳艳玲,等.少根紫萍转录因子及其营养胁迫下的表达[J].应用与环境生物学报,2018,24(01):97-101.[doi: 10.19675/j.cnki.1006-687x.2017.04015]
 LI Zhidan,FANG Yang,et al.Transcription factors and their expression in $Landoltia punctata$ under nutrient starvation[J].Chinese Journal of Applied & Environmental Biology,2018,24(01):97-101.[doi: 10.19675/j.cnki.1006-687x.2017.04015]
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少根紫萍转录因子及其营养胁迫下的表达()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年01期
页码:
97-101
栏目:
研究论文
出版日期:
2018-02-09

文章信息/Info

Title:
Transcription factors and their expression in $Landoltia punctata$ under nutrient starvation
作者:
李志丹方扬靳艳玲丁彦强何开泽李燕王琼瑶赵海
1中国科学院环境与应用微生物重点实验室 成都 610041 2中国科学院成都生物研究所 成都 610041 3中国科学院大学 北京 100049 4四川省自然资源科学研究院 成都 610041
Author(s):
LI Zhidan FANG YangJIN YanlingDING Yanqiang HE KaizeLI Yan WANG QiongyaoZHAO Hai**
1 Key Laboratory of Environment and Applied Microbiology, Chinese Academy of Sciences, Chengdu 610041, China 2 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 3 University of Chinese Academy of Sciences, Beijing 100049, China 4 Sichuan Provincial Academy of Natural Resource Sciences, Chengdu 610041, China
关键词:
少根紫萍转录因子营养胁迫转录组
Keywords:
Landoltia punctata transcription factor nutrient starvation transcriptome
分类号:
Q78 : Q945.79
DOI:
10.19675/j.cnki.1006-687x.2017.04015
摘要:
少根紫萍(Landoltia punctata)是一种浮水生、多功能、高环境适应能力的能源植物,为了解其生理特性的分子机制,利用少根紫萍转录因子预测数据与拟南芥、水稻、玉米数据库数据进行宏观比较,并结合转录组测序技术对少根紫萍营养胁迫后转录因子表达分析. 结果显示,少根紫萍有1 076个转录因子,分属于66个家族,其中在bZIP、WRKY、AP2/ERF-ERF、MYB、NAC、MADS-box等家族基因数明显减少,一定程度上解释了浮萍高黄铜低木质素、难开花的生理特性;少根紫萍在营养胁迫下偏向上调AP2/ERF-ERF、MYB、bHLH家族特定基因和下调AP2/ERF-ERF、WRKY家族特定基因来响应营养胁迫,尤其是AP2/ERF-ERF、WRKY两个家族中特定基因表达下调在响应水体营养胁迫中有非常重要的作用. 本研究在转录因子层面对浮萍的生理特性,特别针对营养胁迫下转录因子的表达进行了探究,可为建立浮萍水生模式植物系统、深入探讨少根紫萍响应营养胁迫机制以及改造成耐受胁迫的高效淀粉积累能源植物提供理论指导. (图2 表4 参33)
Abstract:
Landoltia punctata (duckweed) is a type of floating plant with multiple functions and high environmental adaptability. Research on the molecular mechanisms of its physiological characteristics is scarce. Transcription factors (TFs), as essential gene expression regulation trans-acting factors, play a key role in plant physiological metabolism. This study aimed to uncover the macroscopic differences in TFs associated with the characteristics of L. punctata in comparison with other plants, to investigate the role of TFs in responding to nutrient starvation, and finally, to guide its large-scale applications. The TFs of L. punctata were predicted using iTAK (a plant TF,protein kinase identifier and classifier) and then compared with those of Arabidopsis thaliana, Oryza sativa, and Zea mays. Meanwhile, the TFs of L. punctata under nutrient starvation were analyzed at three time points: 0, 2, and 24 h. The results showed that approximate 1 076 transcription factors belonging to 66 families exist in L. punctata. The levels of AP2/ERF-ERF, B3, bHLH, bZIP, GeBP, C2H2, GRAS, HB-HD-ZIP, HSF, MADS, MYB, NAC, and WRKY significantly decreased relative to those of A. thaliana, O. sativa, and Z. mays. In addition, the transcriptome analysis of L. punctata under nutrient starvation revealed that certain specific TFs in the AP2/ERF-ERF, MYB, and bHLH families were upregulated, while others, particularly the AP2/ERF-ERF and WRKY families, were downregulated. The comparative analysis of TFs implied that the decline in NAC and MADS-box TFs might account for the high-flavone levels accompanied by low-lignin content and the rare flowering of duckweed, respectively. The results of the present study provide the basis for further molecular research in L. punctate and show that L. punctata could serve as a model plant in the future.

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更新日期/Last Update: 2018-02-09