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Microbial diversity in Yubei Oil field determined by high-throughput sequencing(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

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Microbial diversity in Yubei Oil field determined by high-throughput sequencing
GU Lei1 2 XU Kewei2 TANG Yuping2 YANG Fan2 & SUN Yongge1**
1Department of Earth Science, Zhejiang University, Hangzhou 310027, China 2Wuxi Research Institute of Petroleum Geology, Research Institute of Petroleum Exploration & Production, SINOPEC, Wuxi 214151, China
microbial prospecting for oil and gas high-throughput sequencing community structure microbial diversity
Q939.99 : TE357.9

Identification of active microbial markers is the core of microbial prospecting for oil and gas. However, current studies lack an in-depth understanding of oil and gas microbial markers above the typical reservoirs. In order to further understand the microbial information above the oil and gas fields, the microbial diversity in the soil above the Yubei oil field was studied. In this study, high-throughput sequencing techniques were used to investigate the abundance of oil and gas microbial species and community structure in the Yubei area. The results showed that samples obtained above the oil and gas spots contained more endemic species than those from obtained from the background non-oil and gas spots; 199 different microbes were present in the oil and gas samples compared with 77 different microbes in the non-oil and gas samples. Ten major oil-gas microbes were identified, and three dominant species (Pseudomonas, Bacillus, and Agrobacterium) are facultative microbial organisms. Furthermore, two potential families of microbial indicator bacteria (Vibrionaceae and Erythrobacteraceae) were identified. Based on the abnormal distribution of oil and gas microbes, an oil and gas enrichment zone and potential favorable drilling targets are inferred in the Yubei area. The results of oil and gas microbe in Yubei area with microbial distribution of the characteristics in this study laid the foundation, in order to further study indicator bacteria for underlying oil and gas.


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Last Update: 2017-04-25