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Overview and comparison of research methods for determining the community structure and diversity of arbuscular mycorrhizal fungi(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

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Overview and comparison of research methods for determining the community structure and diversity of arbuscular mycorrhizal fungi
LIN Xiangui1** HU Junli1 DAI Jue1 2 WANG Fayuan1 3 & FENG Youzhi1
1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China 2Shanghai Cooperative Centre for WEEE Recycling, School of Environmental and Materials Engineering, Shanghai Polytechnic University, Shanghai 201209, China 3College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao 266045, China
arbuscular mycorrhizal fungi spore morphological identification PCR-DGGE 454 pyrosequencing
Q93-3 : S154.36

Investigating the community structure and diversity of arbuscular mycorrhizal (AM) fungi is important for accelerating the protection and application of mycodiversity, and for the sustainable development of ecosystems. Nevertheless, establishment of related research methods is the key to realizing and promoting such investigations. In this paper, we first provide a very brief historical survey on research methods of AM fungi, and introduce the principles and steps of both morphological and molecular biological methods, including spore morphological identification, polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), and 454 pyrosequencing. Then, the results related to the long-term fertilization effects on soil AM fungal community structure and diversity revealed by spore calculation and identification, real-time PCR and PCR-DGGE, and 454 pyrosequencing were integrated for method comparison. Altogether, different methods could not only complement each other or have effects of mutual corroboration, but also have in consistencies and contradictions. Finally, the future development of research methods for AM fungi are proposed, including an upgrade of the pyrosequencing technique, and its combined application with biomarker methods, isotope labeling techniques, and others.


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Last Update: 2017-04-25