|Table of Contents|

Determination of antibiotic activity of myxobacteria and their correlation with environmental factors(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2017年第2期
Page:
244-250
Research Field:
Articles
Publishing date:
2017-04-25

Info

Title:
Determination of antibiotic activity of myxobacteria and their correlation with environmental factors
Author(s):
WU Zhihua DING Yixiu REN Xingbo ZHAO Puyu SU Lite & LIU Huirong**
College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010010, China
Keywords:
myxobacteria isolation identification antibiotic activity environmental parameter correlation
CLC:
Q938 + Q939.9
PACS:
DOI:
10.3724/SP.J.1145.2016.03024
DocumentCode:

Abstract:
The aim of this study was to evaluate the antibiotic activity of myxobacteria and analyze the relationship between environmental factors and the distribution of myxobacteria. Myxobacteria were isolated from 20 soil samples by the rabbit dung pellets-inducing method, Escherichia coli-inducing method, and filter paper-inducing method. The purified strains were identified by morphological observation, physiological and biochemical characteristics, and 16S rDNA sequence analysis. Then, the antibiotic activities of the strains were determined by the plate confrontation method. The correlation of the distribution of the strains with environmental factors was analyzed by statistical analysis. A total of 140 strains were isolated in this experiment and 39 of them were purified. The identification results showed that these strains belonged to the genus Myxococcus, including M. xanthus (17 strains), M. flavescens (13 strains), M. virescens (5 strains), and 4 unidentified strains. Antibiotic activity tests indicated that all of the purified myxobacteria strains exhibited one or more kinds of antibiotic activities. Thirty-seven strains showed activity against E. coli, 35 strains showed activity against Phytophthora infestans, 26 strains showed activity against Staphylococcus aureus, 20 strains showed activity against Saccharomyces cerevisiae, and 19 strains showed activity against Bacillus subtilis. Analysis of the relationship between environmental factors and the distribution of myxobacteria indicated that the number of bacteriolytic myxobacteria, the number of celluloytic myxobacteria, and even the total number of strains had positive correlations with pH value and available potassium, and had negative correlations with the water content; no significant correlations were detected with the other parameters. Myxococcus is the dominant genus in the area of Liangcheng in Inner Mongolia, China, and the vast majority of the myxobacterial strains isolated from this region show different levels of antibiotic activities. This work contributes to enriching the myxobacterial resources in Inner Mongolia and will lay a foundation for further research on myxobacteria for the discovery and development of new agricultural chemicals.

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Last Update: 2017-04-25