|Table of Contents|

A quantitative proteomics study of the inhibitory effect of D. denneanum on lung tumor cells and its mechanism(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2017 01
Research Field:
Publishing date:


A quantitative proteomics study of the inhibitory effect of D. denneanum on lung tumor cells and its mechanism
ZHANG Hua1 2 LI Ying1 2 LIU Jing1 HE Tao1 JIANG Wei1 CHUN Ze1** & HU Yadong1**
1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2University of Chinese Academy of Sciences, Beijing 100049, China
D. denneanum lung cancer cells mechanism of anti-tumor SILAC quantitative proteomics

Dendrobium denneanum is shown to have significant anti-cancer effects, but their specific anti-cancer mechanism is still not understood in detail. Because of the complex composition of its extract, traditional research methods could not find out the anticancer mechanism and its targets systematically. To solve this problem, this research aimed to study the anticancer effect and mechanism of ethanol extract of D. denneanum based on quantitative proteomics. We used quantitative proteomics based on SILAC technique to investigate the inhibitory function of D. denneanum on lung tumor cell line A549 and the mechanism underlying such effect. The result showed that ethanol extract of D. denneanum not only significantly inhibited cell proliferation of A549 cell line, but also led to cell death. The inhibition rate reached 35.05% at 24 h and 59.08% at 48 h. Quantitative proteomics experiments found that 392 proteins of A549 cell line were down regulated by the ethanol extract of D. denneanum. Function analysis showed that the down-regulated proteins were mainly those involved in cellular material and energy metabolism, such as RNA metabolism and protein synthesis, carbohydrate metabolism, lipid metabolism, purine and pyrimidine metabolism; a large number of proteins with the function of resisting endogenous and external adverse stimuli and injuries including redox molecular chaperones, DNA repair, protein degradation and restoration, were also significantly down regulated. This research explains from the point of quantitative proteomics the cancer inhibition effect and its mechanism of D. denneanum, and lays foundation for future research and development of new anticancer drugs based on D. denneanum.


1 Guo P, Huang ZL, Yu P. Trends in cancer mortality in China: an update [J]. Ann Oncol Offic JEur Soc Med Oncol, 2012, 23 (10): 2755-2762
2 Ng TB, Liu J, Wong JH. Review of research on Dendrobium, a prized folk medicine [J]. Appl Microbiol Biotechnol, 2012, 93 (5): 1795-1803
3 Jae In S, Yu Jin K, Hae-Young Yl. Denbinobin, a phenanthrene from Dendrobium nobile, inhibits invasion and induces apoptosis in SNU-484 human gastric cancer cells [J]. Oncol Rep, 2011, 27 (3): 813-818
4 An-Chi T, Shiow-Lin P, Cho-Hwa L. Moscatilin, a bibenzyl derivative from the India orchid Dendrobrium loddigesii, suppresses tumor angiogenesis and growth in vitro and in vivo [J]. Cancer Lett, 2010, 292 (2): 163-170
5 Prasad R, Koch B. Antitumor activity of ethanolic extract of Dendrobium formosum in T-cell lymphoma: an in vitro and in vivo study [J]. Biomed Res Intern, 2014, 2014 (6): 753451-753451
6 罗傲雪, 宋关斌, 范益军. 迭鞘石斛抗肿瘤作用动物实验研究[J]. 四川大学学报自然科学版, 2005, 42 (6): 1281-1283 [Luo AX, Song GB, Fan YJ. The research on the inhibiting tumor effect of D. Denneanum in vivo [J]. J Sichuan Univ (Nat Sci Ed ), 2005, 42 (6): 1281-1283]
7 罗傲雪, 宋关斌, 淳泽, 秦建, 罗傲霜, 范益军, 何涛. 迭鞘石斛抗肿瘤作用研究[J]. 应用与环境生物学报, 2007, 13 (2): 184-187 [Luo AX, Song GB, Chun Z, Qin J, Luo AS, Fan YJ, He T. Inhibiting effect of tumor by Dendrobium denneanum. Chin J Appl Environ Biol, 2007, 13 (2): 184-187]
8 Christofk HR, Heiden MGV, Harris MH. The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth [J]. Nature, 2008, 452 (7184): 230-233
9 Yan G, Wei J, Lu G. Mouse embryonic fibroblasts from CD38 knockout mice are resistant to oxidative stresses through inhibition of reactive oxygen species production and Ca (2+) overload [J]. BBRC, 2010, 399 (2): 167-172
10 Stuart JA, Mayard S, Hashiguchi K. Localization of mitochondrial DNA base excision repair to an inner membrane-associated particulate fraction [J]. Nucleic Acids Res, 2005, 33 (12): 3722-3732
11 Rossi MR, Seema S, Garrett SH. Expression of hsp 27, hsp 60, hsc 70, and hsp 70 stress response genes in cultured human urothelial cells (UROtsa) exposed to lethal and sublethal concentrations of sodium arsenite [J]. EnvironHealth Perspect, 2002, 110 (12): 1225-1232
12 Torkild V, Berit D, Henrik Sahlin P. Uracil in DNA and its processing by different DNA glycosylases [J]. Philos Trans Royal Soc Lond, 2009, 364 (1517): 563-568
13 Rhee SG, Chae HZ, Kim K. Peroxiredoxins: a historical overview and speculative preview of novel mechanisms and emerging concepts in cell signaling [J]. Free Radical Biol Med, 2005, 38 (12): 1543-1552
14 Hyun AeW, Hee YS, Hae SD. Inactivation of peroxiredoxin I by phosphorylation allows localized H(2)O(2) accumulation for cell signaling [J]. Cell, 2010, 140 (4): 517-528
15 Egler RA, Fernandes EK, Sereika S. Regulation of reactive oxygen species, DNA damage, and c-Myc function by peroxiredoxin 1 [J]. Oncogene, 2005, 24 (54): 8038-8050
16 Joo Young H, Yunghee K, Jaeho J. Peroxiredoxin 3 is a key molecule regulating adipocyte oxidative stress, mitochondrial biogenesis, and adipokine expression [J]. Antioxid Redox Signal, 2012, 16 (3): 229-243
17 Wasinger VC, Zeng M, Yau Y. Current Status and Advances in Quantitative Proteomic Mass Spectrometry [J]. Int J Proteomics, 2013, 2013 (2013): 180605-180605
18 Matthias M. Functional and quantitative proteomics using SILAC [J]. Nat Rev Mol Cell Biol, 2006, 7 (12): 952-958


Last Update: 2017-02-25