|Table of Contents|

A quantitative proteomics study of the inhibitory effect of D. denneanum on lung tumor cells and its mechanism(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2017 01
Page:
100-104
Research Field:
Articles
Publishing date:

Info

Title:
A quantitative proteomics study of the inhibitory effect of D. denneanum on lung tumor cells and its mechanism
Author(s):
ZHANG Hua1 2 LI Ying1 2 LIU Jing1 HE Tao1 JIANG Wei1 CHUN Ze1** & HU Yadong1**
1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2University of Chinese Academy of Sciences, Beijing 100049, China
Keywords:
D. denneanum lung cancer cells mechanism of anti-tumor SILAC quantitative proteomics
CLC:
R285.5
PACS:
DOI:
10.3724/SP.J.1145.2016.02006
DocumentCode:

Abstract:
Dendrobium denneanum is shown to have significant anti-cancer effects, but their specific anti-cancer mechanism is still not understood in detail. Because of the complex composition of its extract, traditional research methods could not find out the anticancer mechanism and its targets systematically. To solve this problem, this research aimed to study the anticancer effect and mechanism of ethanol extract of D. denneanum based on quantitative proteomics. We used quantitative proteomics based on SILAC technique to investigate the inhibitory function of D. denneanum on lung tumor cell line A549 and the mechanism underlying such effect. The result showed that ethanol extract of D. denneanum not only significantly inhibited cell proliferation of A549 cell line, but also led to cell death. The inhibition rate reached 35.05% at 24 h and 59.08% at 48 h. Quantitative proteomics experiments found that 392 proteins of A549 cell line were down regulated by the ethanol extract of D. denneanum. Function analysis showed that the down-regulated proteins were mainly those involved in cellular material and energy metabolism, such as RNA metabolism and protein synthesis, carbohydrate metabolism, lipid metabolism, purine and pyrimidine metabolism; a large number of proteins with the function of resisting endogenous and external adverse stimuli and injuries including redox molecular chaperones, DNA repair, protein degradation and restoration, were also significantly down regulated. This research explains from the point of quantitative proteomics the cancer inhibition effect and its mechanism of D. denneanum, and lays foundation for future research and development of new anticancer drugs based on D. denneanum.

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